The DNA replication group, Imperial College London, is looking to recruit a motivated and highly creative Research Associates interested in understanding how the eukaryotic replicative helicase promotes accurate DNA replication using biochemical approaches.
One of the most fundamental property of all living things is the ability to replicate their DNA, which allows cells to pass on their genetic material from parent to progeny. The Speck’s lab reconstituted eukaryotic replicative helicase loading with purified Saccharomyces cerevisiae proteins and identified the MCM2-7 double-hexamer as the template for replication fork assembly (Evrin et al PNAS 2009).
We are now in a strong position to address how the replicative helicase becomes activated for DNA synthesis.
The candidates will employ our well-established in vitro reconstituted system (Evrin et al PNAS 2009; Noguchi et al PNAS 2017) to study how the replicative helicase becomes activated during S-phase.
Duties and responsibilities
- To generate reagents for the project
- To design and carry out scientific experiments in accordance with the project
- To maintain highly organised and accurate records of experimental work
- To prepare material for presentation in oral and poster formats and present findings to colleagues and at conferences
- To contribute to writing bids for research grants
- To analyse, manage and communicate data, ensuring the validity and reliability of the data at all times
- To draft publications and prepare them for submission to refereed journals
- To submit publications to refereed journals
- To actively contribute to the smooth running of the laboratory in co-ordination with other members of the group
- To form a good working relationship with other members of the group and actively participate in team work
- To promote the reputation of the group, Centre, the Department and the College
- To develop contacts and research collaborations within the Centre, the College and the wider community
- To supervise staff, as appropriate, and to provide guidance and occasional supervision to junior members of the laboratory, if requested
- To actively participate in the group’s research meetings and academic activities organised by the Institute (including internal seminars, lectures, institute retreats and public-related activities)
- Research Associate: Hold a PhD (or equivalent) in Epidemiology or medical statistics or a closely related discipline
- Research Assistant: Near completion of a PhD (or equivalent) in Epidemiology or medical statistics or a closely related discipline
- Epidemiology or medical statistics or a closely related discipline
- Have excellent experience in protein biochemistry of replication factors, biochemical assays and the preparation of samples for cryo-EM analysis
- At least one first-author publication in an internationally recognised peer-reviewed journal (published)
- Strong experience in preparing samples for cryo-EM
- Strong experience in protein expression, purification of replication factors
- Solid knowledge in working with purified proteins
This post is based at the Hammersmith Campus in London for a period of 6 months.
Candidates who have not yet been officially awarded their PhD will be appointed as a Research Assistant within the salary range £36,045 – £39,183 per annum
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Please note that job descriptions cannot be exhaustive and the post-holder may be required to undertake other duties, which are broadly in line with the above key responsibilities.
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