This role is primarily to manage a number of transgenic mouse lines modelling aspects of Amyotrophic Lateral Sclerosis. The aim of the project is to carry out a longitudinal study including behavioural characterisation and tissue banking for cellular and molecular investigation using standard methodologies.
Additional duties will comprise support with colony maintenance and tissue harvesting of additional transgenic mouse lines. The role will involve extensive mouse handling, behavioural phenotyping, and taking and banking tissue for downstream immunohistochemistry, western blotting and microscopy studies. An important aspect is regular genotyping of mouse biopsies via DNA extraction, PCR (polymerase chain reaction) and restriction digest to determine transgenic status. Therefore, some basic molecular biology experience would be valuable as would a working knowledge of neurobiology and mouse anatomy.
Current or very recent experience with mouse husbandry and handling in addition to possessing an up to date UK animal training certificate (PIL A and B) is essential.
As this is a part time position (0.6 FTE, 21 hours a week), work hours are open to flexibility.
This post will be offered on an a fixed-term contract up to 28/02/2023 with the possibility of a further extension in due course.
This is a part-time post – 0.6 FTE
- Weekly management, husbandry and longitudinal monitoring of mouse colonies
- Regular behavioral phenotyping using rotarod and grip strength meter and other tests where required
- Culling, perfusion, snap freezing and sectioning of cortex and spinal cord tissues for downstream experimental procedures and investigation
- Genotyping of mice using DNA extraction, PCR and restriction digest
- Maintenance and record keeping of mouse colonies using Microsoft Office and online, in-house King’s databases
- Regular meetings and communication with line managers and researchers on the project.
The above list of responsibilities may not be exhaustive, and the post holder will be required to undertake such tasks and responsibilities as may reasonably be expected within the scope and grading of the post.
Skills, knowledge, and experience
- Mouse handling, working with mouse tissue including perfusion and fixing
- Behavioural testing experience ie. rotarod and grip strength
- Assist with tissue provision for researchers
- Upkeep of databases
- BSc degree (or equivalent)
- Mouse handling and behavioral testing experience in a research setting
- Experience of working with mouse tissue
- Up to date UK animal training certificate (PIL A and B)
- Basic molecular biology skills ie. DNA extraction and PCR.
- Rigorous attention to detail
- Diligent and communicative
- Ability to work independently and in a team
- Knowledge of neurodegenerative disease and mouse anatomy