We are a world class research-intensive university. We deliver teaching and learning of the highest quality. We play a leading role in economic, social and cultural development of the North East of England. Attracting and retaining high-calibre people is fundamental to our continued success.
Research Assistant: £28,756 – £29,614, rising to £30,497 per annum
Research Associate: £31,406, rising to £40,927 per annum
Closing Date: 17 February2022
This British Heart Foundation funded project aims to identify the genetic regulatory networks within cells that contribute to the development of the aortic arch arteries. This research follows on from our recently published work that identified genetic interactions between Pax9, Tbx1, Gbx2 and Msx1 in the pharyngeal tissues (PMID: 31444215, PMID: 32466118 and PMID: 34615475). Your role will be to optimise and perform nuclei extractions from mammalian embryonic tissue for single cell RNA-seq analysis. Following data analysis key candidate genes will be further analysed in model organisms.
A scientific background in cardiovascular development will be essential and applicants with a record of accomplishment and expertise in single cell transcriptomics are preferred.
The project is a collaboration between research groups at Newcastle University and the University of Manchester.
Find out more about the Faculty of Medical Sciences here: https://www.ncl.ac.uk/medical-sciences/
Find out more about our Research Institutes here:
This post is fixed term for a period of 2 years.
For informal enquiries contact: Dr Simon Bamforth (firstname.lastname@example.org).
• Although working under the general guidance of an academic or Principal Investigator, the postholder will contribute ideas, including enhancements to the technical or methodological aspects of their studies, thus providing substantial ‘added value’
• Develop and carry out the specified project using appropriate techniques and equipment as outlined in the personal requirements
• Determine appropriate methodologies for research, with advice and support where required
• Contribute to grant applications submitted by others and in time develop own research objectives and proposals for funding
• Begin to write, with appropriate support, proposals for individual research funding or, where funders do not permit this, contribute to the writing of collective bids
• Assess research findings for the need/scope for further investigations
• Contribute to the writing up of their research for publication and dissemination, either through seminar and conference presentations or through publications
• Present research findings, either at conferences or through publications in reputable outlets appropriate to the discipline
• May be involved in the supervision, with guidance, of final year undergraduate research projects and in providing support to postgraduate research students or Research Assistants
• Will need to work with the support staff and, on occasions, with undergraduate and postgraduate students, and interact intellectually with other academic members of the Institute
• May contribute to events celebrating the public engagement of science/social sciences/humanities
• Develop an awareness of University structures, policies and procedures and relevant issues in the higher education, research, social and political environment
• Perform micro-dissection of tissue samples for analysis
• Liaise with the Flow Cytometry and Genomics core facilities to coordinate sample preparation for single cell RNA-seq.
• Interact with the core Bioinformatics facility in analysing single cell RNA-seq data
• Validate the results of transcriptomic analysis in model organisms
Knowledge, Skills and Experience
• Ability to work well as part of a team and rapidly acquire new skills
• Detailed subject knowledge in the area of research
• Likelihood of advanced skills directly related to the research projects
• High level of analytical and problem-solving capability
• Ability to communicate complex information with clarity and to encourage the commitment of others
• Experience of research with clear transferable skills and some experience or awareness of the research environment
• Presentations at conferences and/or high-quality publications
• Knowledge of cardiovascular development
• Excellent generic laboratory skills and evidence of ability to deliver technically difficult experiments
• Expertise in molecular biology (e.g. primer design, PCR, quantitative real-time RT-PCR, cloning)
• Experience in embryo tissue processing, flow cytometry, immunohistochemistry and in situ hybridisation
• Mouse embryo analysis (including breeding, embryo collection)
• Expertise in single cell transcriptomics
• Evidence of a record of research including publication and presentation of data at conferences
• Home Office personal licence for animal handling
Attributes and Behaviour
• High level laboratory skills
• Attention to detail
• Able to analyse and interpret data
• Strong critical thinking skills
• Evidence of good oral communication skills; flexible independent working; initiative; and ability to work as part of a multi-disciplinary team
• Excellent interpersonal skills
• Able to prioritise key tasks and meet deadlines
• Willingness to work outside normal hours and weekends as necessary
• Highly motivated and enthusiastic
• Experience of supervision of junior staff members
• Experience of managing/organising a lab
• Experience of producing laboratory protocols and risk assessments
• Good honours degree (or equivalent) with some subject knowledge in the relevant area (Research Assistant)
• A PhD in the research area required (Research Associate)
Newcastle University is committed to being a fully inclusive Global University which actively recruits, supports and retains colleagues from all sectors of society. We value diversity as well as celebrate, support and thrive on the contributions of all our employees and the communities they represent.We are proud to be an equal opportunities employer and encourage applications from everybody, regardless of race, sex, ethnicity, religion, nationality, sexual orientation, age, disability, gender identity, marital status/civil partnership, pregnancy and maternity, as well as being open to flexible working practices.
The University holds a silver Athena SWAN award in recognition of our good employment practices for the advancement of gender equality. The University also holds the HR Excellence in Research award for our work to support the career development of our researchers, and is a member of the Euraxess initiative supporting researchers in Europe.
We understand how important the full employment package is to our colleagues at Newcastle University and we are committed to providing a great range of benefits and discounts for all. You can learn more about what is available here on ourBenefitsWebsite page.
Requisition ID: 15843