A 3-year PhD Studentship funded by Great Ormond Street Hospital Charity is available in the Infection, Immunity and Inflammation Research and Teaching Department, Great Ormond Street UCL Institute of Child Health. The studentship will commence in March 2022 under the supervision of Professor Paul Brogan (primary supervisor), and Professor Despina Eleftheriou.
The aim of the studentship is to examine molecular genetics of patients with autoinflammation and vasculitis, devise tailored novel assays to dissect pathogenesis and formulate these findings into a precision medicine framework for autoinflammation. The student will:
(i) learn whole exome sequencing: in silico scrutiny of a targeted gene panel based on data derived from whole exome, then if no obvious answer move onto true gene discovery (novel gene identification) using established and well worked up pipelines;
(ii) exploration of software pipelines linking phenotype and genotype (e.g. exomiser) to facilitate novel gene discovery;
(iii) explore whole genome sequencing if whole exome sequencing does not provide the answer, but the genetic cause is still suspected;
(iv) develop, refine and validate functional readouts of pathways linked to genotypes identified in individual cases from GOSH, known to lead to autoinflammation.
Pertinent pathways include: inflammasomes, proteasomes, type I interferonopathy, ADA2, complementopathies, interferon gamma and other HLH related pathways, amongst others). In cases where functional read outs are not clearly developed yet the student will learn how to use: cellular protein expression with immunoblot or flow cytometry, mRNA with qPCR followed by siRNA silencing of CRISPR/CAS9 gene editing and gene rescue experiments in pertinent cells (cell lines and primary human cells where available) to examine impact on inflammatory pathways and devise new assays. The PhD student will also obtain generic skills by attending relevant courses run by UCL.
- Project Description
The student will receive a starting stipend of 17,764 per annum (including London weighting) as well as the cost of tuition fees based on UK fee status.
Applicants should have a good first degree (UK 1st class or upper 2nd class honours degree or equivalent from abroad) and a Masters degree (or equivalent work experience) in a relevant discipline by the time of registration.
This studentship covers the cost of tuition fees based on the UK (Home) rate. Non-UK students can apply but will have to personally fund the difference between the UK (Home) rate and the overseas rate where they are not eligible for UK fee status.
NB: You will be asked about your likely fee status at the interview so we would advise you to contact the UCL Graduate Admissions Office for advice should you be unsure whether or not you meet the eligibility criteria for Home fee status. Further information on Brexit and the definitions for fee status assessment can be found on the UCL website and also the UKCISA website (Higher Education: Definitions for fee status assessment).
To apply, please send a current CV including the contact details of two professional referees as well as a cover letter to firstname.lastname@example.org; enquiries about the post can be made to Prof Paul Brogan: email@example.com.
Professor Paul Brogan
UCL Taking Action for Equality
10 Dec 2021
Latest time for the submission of applications
10th January 2022
Studentship Start Date